A method for the preparation of heavy-atom derivatives of yeast cytochrome c peroxidase.
نویسندگان
چکیده
This Letter describes a strategy for preparation of heavy-atom derivatives which has succeeded with yeast cytochrome c peroxidase and which may be useful for other crystalline proteins. Transfer of the crystals from methylpentanediol solutions, in which they were grown, to solutions of polyethylene glycol (34, 6000) maintains the crystal structure so that crystals tolerate heavy-atom concentrations at least ten times larger than could be attained in methylpentanediol mother liquors.
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عنوان ژورنال:
- Journal of molecular biology
دوره 127 2 شماره
صفحات -
تاریخ انتشار 1979